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drp1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc drp1
    A , gene expression of fission and fusion markers Fis1, <t>Drp1,</t> OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
    Drp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 946 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+drp1/DRP1+Rabbit+mAb/pmc13674193-101-7-8
    Average 97 stars, based on 946 article reviews
    drp1 - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia"

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    Journal: The Journal of Physiology

    doi: 10.1113/JP291356

    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
    Figure Legend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Techniques Used: Gene Expression, Western Blot, Expressing

    Related Articles

    Western Blot:

    Article Title: Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism
    Article Snippet: The membranes were then washed with TBST and incubated with secondary indicator antibody (IRDye secondary antibodies, LI-COR, 1:3000) in 5% milk protein/TBST with 0.01% SDS at room temperature for 1 h. After washing with TBST 3 times, the membranes were then scanned by the Odyssey CLx imaging system with Image Studio software version 6.1 (LI-COR, Lincoln, NE, USA). .. Primary antibodies used for Western blots were anti-Mitofilin (Mic60 or Immt) (#10179-1-AP, Proteintech, Rosemont, IL, USA, 1:1000), anti-Mic19 (#25625-1-AP, Proteintech, 1:1000), anti-Sam50 (#28679-1-AP, Proteintech, 1:1000), anti-PINK1 (#BC100-494, Novus Biologicals, Centennial, CO, USA, 1:1000), anti-LC3 (#3868, Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-p62/SQSTM1 (#P0067, Sigma Aldrich, 1:1000), anti-LAMP2 (#ABL-93, Developmental Studies Hybridoma Bank, 1:50), anti-LIMPII (#ab176317, abcam, Cambridge, UK, 1:1000), anti-PGC1α (#ab54481, abcam, 1:1000), anti-PGC1β (#ab176328, abcam 1:1000), anti-MFN2 (#9482, Cell Signaling Technology, 1:1000), anti-MFN1 (#ab104274, abcam, 1:750), anti-Ubiquitin (#sc-8017, Santa Cruz, Dallas, TX, USA, 1:200), anti-GAPDH (#10R-G109A, Fitzgerald, Acton, MA, USA, 1:5000), anti-Mic26 (#MA5-15493, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000), antiMic27 (#28514-1-AP, Proteintech, 1:1000), anti-Mic10 (#31561-1-AP, Proteintech, 1:500), anti-SESN2 (#10795-1-AP, Proteintech, 1:1000), anti-cGas (#31659, Cell Signaling Technology, 1:1000), anti-cMYC (#10828-1-AP, Proteintech, 1:2000), anti-GSDMA (#30354-1-AP, Proteintech, 1:2000), anti-OPTN (#10837-1-AP, Proteintech, 1:5000), anti-OPA1 (#ab157457, abcam, 1:1000), and anti-DRP1 (#8570, Cell Signaling Technology, 1:1000). .. Total RNA was isolated with TRIzol reagent (#15596018, Thermo Fisher Scientific) from heart tissue as previously described [25].

    Article Title: Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism
    Article Snippet: The membranes were then washed with TBST and incubated with secondary indicator antibody (IRDye secondary antibodies, LI-COR, 1:3000) in 5% milk protein/TBST with 0.01% SDS at room temperature for 1 h. After washing with TBST 3 times, the membranes were then scanned by the Odyssey CLx imaging system with Image Studio software version 6.1 (LI-COR, Lincoln, NE, USA). .. Primary antibodies used for Western blots were anti-Mitofilin (Mic60 or Immt) (#10179-1-AP, Proteintech, Rosemont, IL, USA, 1:1000), anti-Mic19 (#25625-1-AP, Proteintech, 1:1000), anti-Sam50 (#28679-1-AP, Proteintech, 1:1000), anti-PINK1 (#BC100-494, Novus Biologicals, Centennial, CO, USA, 1:1000), anti-LC3 (#3868, Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-p62/SQSTM1 (#P0067, Sigma Aldrich, 1:1000), anti-LAMP2 (#ABL-93, Developmental Studies Hybridoma Bank, 1:50), anti-LIMPII (#ab176317, abcam, Cambridge, UK, 1:1000), anti-PGC1α (#ab54481, abcam, 1:1000), anti-PGC1β (#ab176328, abcam 1:1000), anti-MFN2 (#9482, Cell Signaling Technology, 1:1000), anti-MFN1 (#ab104274, abcam, 1:750), anti-Ubiquitin (#sc-8017, Santa Cruz, Dallas, TX, USA, 1:200), anti-GAPDH (#10R-G109A, Fitzgerald, Acton, MA, USA, 1:5000), anti-Mic26 (#MA5-15493, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000), anti-Mic27 (#28514-1-AP, Proteintech, 1:1000), anti-Mic10 (#31561-1-AP, Proteintech, 1:500), anti-SESN2 (#10795-1-AP, Proteintech, 1:1000), anti-cGas (#31659, Cell Signaling Technology, 1:1000), anti-cMYC (#10828-1-AP, Proteintech, 1:2000), anti-GSDMA (#30354-1-AP, Proteintech, 1:2000), anti-OPTN (#10837-1-AP, Proteintech, 1:5000), anti-OPA1 (#ab157457, abcam, 1:1000), and anti-DRP1 (#8570, Cell Signaling Technology, 1:1000). .. Total RNA was isolated with TRIzol reagent (#15596018, Thermo Fisher Scientific) from heart tissue as previously described [ ].

    Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission
    Article Snippet: Anti-MFF , 84580 , Rabbit , CST , WB (1:1000). .. Anti-DRP1 , 8570 , Rabbit , CST , WB (1:1000); IF (1:100). .. Anti-DRP1 , 184248 , Rabbit , Abcam , WB (1:1000); IHC (1:2000).

    Cell-Signaling:

    Article Title: Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism
    Article Snippet: The membranes were then washed with TBST and incubated with secondary indicator antibody (IRDye secondary antibodies, LI-COR, 1:3000) in 5% milk protein/TBST with 0.01% SDS at room temperature for 1 h. After washing with TBST 3 times, the membranes were then scanned by the Odyssey CLx imaging system with Image Studio software version 6.1 (LI-COR, Lincoln, NE, USA). .. Primary antibodies used for Western blots were anti-Mitofilin (Mic60 or Immt) (#10179-1-AP, Proteintech, Rosemont, IL, USA, 1:1000), anti-Mic19 (#25625-1-AP, Proteintech, 1:1000), anti-Sam50 (#28679-1-AP, Proteintech, 1:1000), anti-PINK1 (#BC100-494, Novus Biologicals, Centennial, CO, USA, 1:1000), anti-LC3 (#3868, Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-p62/SQSTM1 (#P0067, Sigma Aldrich, 1:1000), anti-LAMP2 (#ABL-93, Developmental Studies Hybridoma Bank, 1:50), anti-LIMPII (#ab176317, abcam, Cambridge, UK, 1:1000), anti-PGC1α (#ab54481, abcam, 1:1000), anti-PGC1β (#ab176328, abcam 1:1000), anti-MFN2 (#9482, Cell Signaling Technology, 1:1000), anti-MFN1 (#ab104274, abcam, 1:750), anti-Ubiquitin (#sc-8017, Santa Cruz, Dallas, TX, USA, 1:200), anti-GAPDH (#10R-G109A, Fitzgerald, Acton, MA, USA, 1:5000), anti-Mic26 (#MA5-15493, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000), antiMic27 (#28514-1-AP, Proteintech, 1:1000), anti-Mic10 (#31561-1-AP, Proteintech, 1:500), anti-SESN2 (#10795-1-AP, Proteintech, 1:1000), anti-cGas (#31659, Cell Signaling Technology, 1:1000), anti-cMYC (#10828-1-AP, Proteintech, 1:2000), anti-GSDMA (#30354-1-AP, Proteintech, 1:2000), anti-OPTN (#10837-1-AP, Proteintech, 1:5000), anti-OPA1 (#ab157457, abcam, 1:1000), and anti-DRP1 (#8570, Cell Signaling Technology, 1:1000). .. Total RNA was isolated with TRIzol reagent (#15596018, Thermo Fisher Scientific) from heart tissue as previously described [25].

    Article Title: NSD2 drives triple-negative breast cancer progression via ATAD3A-dependent senescence.
    Article Snippet: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, with limited therapeutic options and dismal prognosis.. Histone methyltransferase NSD2 and its catalytic product H3K36me2 are established drivers of TNBC metastasis; however, the downstream effectors linking epigenetic regulation to cellular senescence remain largely unknown.. This study utilized transcriptomic profiling, ChIP-qPCR, and in vivo xenograft models to define the function and molecular mechanism of the NSD2-ATAD3A axis.

    Article Title: Heart-Specific and Conditional Deletion of the Immt Gene Reveals Its Role in Regulating Mitochondrial Structure and Total Heart Metabolism
    Article Snippet: The membranes were then washed with TBST and incubated with secondary indicator antibody (IRDye secondary antibodies, LI-COR, 1:3000) in 5% milk protein/TBST with 0.01% SDS at room temperature for 1 h. After washing with TBST 3 times, the membranes were then scanned by the Odyssey CLx imaging system with Image Studio software version 6.1 (LI-COR, Lincoln, NE, USA). .. Primary antibodies used for Western blots were anti-Mitofilin (Mic60 or Immt) (#10179-1-AP, Proteintech, Rosemont, IL, USA, 1:1000), anti-Mic19 (#25625-1-AP, Proteintech, 1:1000), anti-Sam50 (#28679-1-AP, Proteintech, 1:1000), anti-PINK1 (#BC100-494, Novus Biologicals, Centennial, CO, USA, 1:1000), anti-LC3 (#3868, Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-p62/SQSTM1 (#P0067, Sigma Aldrich, 1:1000), anti-LAMP2 (#ABL-93, Developmental Studies Hybridoma Bank, 1:50), anti-LIMPII (#ab176317, abcam, Cambridge, UK, 1:1000), anti-PGC1α (#ab54481, abcam, 1:1000), anti-PGC1β (#ab176328, abcam 1:1000), anti-MFN2 (#9482, Cell Signaling Technology, 1:1000), anti-MFN1 (#ab104274, abcam, 1:750), anti-Ubiquitin (#sc-8017, Santa Cruz, Dallas, TX, USA, 1:200), anti-GAPDH (#10R-G109A, Fitzgerald, Acton, MA, USA, 1:5000), anti-Mic26 (#MA5-15493, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000), anti-Mic27 (#28514-1-AP, Proteintech, 1:1000), anti-Mic10 (#31561-1-AP, Proteintech, 1:500), anti-SESN2 (#10795-1-AP, Proteintech, 1:1000), anti-cGas (#31659, Cell Signaling Technology, 1:1000), anti-cMYC (#10828-1-AP, Proteintech, 1:2000), anti-GSDMA (#30354-1-AP, Proteintech, 1:2000), anti-OPTN (#10837-1-AP, Proteintech, 1:5000), anti-OPA1 (#ab157457, abcam, 1:1000), and anti-DRP1 (#8570, Cell Signaling Technology, 1:1000). .. Total RNA was isolated with TRIzol reagent (#15596018, Thermo Fisher Scientific) from heart tissue as previously described [ ].

    other:

    Article Title: MTFR1L protects the heart from Ischemia–Reperfusion injury via dual mitochondrial mechanisms
    Article Snippet: Antibodies used in this study Name RRID Anti-MTFR1L (Atlas Antibodies Cat# HPA027124, RRID:AB_10600211) Anti-GAPDH (Abcam Cat# ab8245, RRID:AB_2107448) Anti-FLAG (Cell Signaling Technology Cat# 14793, RRID:AB_2572291) Anti-Vinculin (Santa Cruz Biotechnology Cat# sc-73264 RRID:AB_1131292) Anti-β-actin (Yeasen Biotech Cat# 30101ES, RRID:AB_3644235) Anti-VDAC1 (Cell Signaling Technology Cat# 4866, RRID:AB_2272627) Anti-AIF (Cell Signaling Technology Cat# 4642, RRID:AB_2224542) Anti-Lamin B (Proteintech Cat# 66095-1-Ig, RRID:AB_11232208) Anti-cTNT (Abcam Cat# ab8295, RRID:AB_306445) Anti-α-actinin (Abcam Cat# ab68194, RRID:AB_3064857) Anti-DRP1 (Cell Signaling Technology Cat# 8570, RRID:AB_109504) Anti-p-DRP1 (Cell Signaling Technology Cat# 3455, RRID:AB_2085352) Anti-OPA1 (Proteintech Cat# 27733-1-AP, RRID: AB_2810292) Anti-MFN1 (Cell Signaling Technology Cat# 14739, RRID:AB_2744531) Anti-MFN2 (Cell Signaling Technology Cat# 11925, RRID: AB_2750893) Anti-MIC60 (Proteintech Cat# 10179-1-AP, RRID: AB_2127193) Anti-MIC25 (Proteintech Cat# 20639-1-AP, RRID: AB_10697667) Anti-MIC19 (Proteintech Cat# 10179-1-AP, RRID: AB_2127193) D ow nloaded from https://spj.science.org on M arch 22, 2026 78 Anti-MIC10 (Proteintech Cat# 25625-1-AP, RRID: AB_2687533) Anti-CHCHD4 (Proteintech Cat# 21090-1-AP, RRID: AB_10734583) Total OXPHOS Rodent Cocktail (Abcam Cat# ab110413, RRID:AB_2629281) Goat anti-Mouse IgG Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-11005, RRID:AB_2534073) Goat anti-Mouse IgG Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-11001, RRID:AB_2534069) Goat anti-Rabbit IgG Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-11012, RRID:AB_2534079) Goat anti-Rabbit IgG Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-11008, RRID:AB_143165) IRDyeR 800CW Goat antiRabbit IgG(H+L) (Li-Cor Biosciences, Cat# 925-32211, RRID: AB_2651127) IRDyeR 800CW Goat antiMouse IgG(H+L) (Li-Cor Biosciences, Cat# 926-32210, RRID: AB_621842) Supplementary Table 3.

    Incubation:

    Article Title: NSD2 drives triple-negative breast cancer progression via ATAD3A-dependent senescence.
    Article Snippet: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, with limited therapeutic options and dismal prognosis.. Histone methyltransferase NSD2 and its catalytic product H3K36me2 are established drivers of TNBC metastasis; however, the downstream effectors linking epigenetic regulation to cellular senescence remain largely unknown.. This study utilized transcriptomic profiling, ChIP-qPCR, and in vivo xenograft models to define the function and molecular mechanism of the NSD2-ATAD3A axis.



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    A , gene expression of fission and fusion markers Fis1, <t>Drp1,</t> OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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    Image Search Results


    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Journal: The Journal of Physiology

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    doi: 10.1113/JP291356

    Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

    Techniques: Gene Expression, Western Blot, Expressing